Single‑cell visual proteomics reveals coordinated gene expression machineries in a minimal bacterium

Cryo‑electron tomography of native and antibiotic‑perturbed Mycoplasma pneumoniae cells yields 140 in‑cell maps capturing translation initiation, elongation, and recycling. The study visualizes transcription‑translation assemblies, proposes a threading‑based reinitiation mechanism, and provides structural evidence for a supercomplex coordinating transcription, translation, and membrane attachment


Read full article for free (open access):

https://www.sciencedirect.com/science/article/pii/S0092867426010652#


Cryo‑electron tomographic visualization of Mycoplasma pneumoniae showing ribosomes, transcription‑translation assemblies, and membrane‑associated large subunits


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