Posts

Vaginal microbiota transfer ameliorates cesarean-associated neurodevelopmental deficits in mice via N-bc2S1P synthesis on neonatal skin

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  Highlights • VMT restores skin  N -bc2S1P linked to early neurodevelopment in cesarean-born neonates • N -bc2S1P engages β-arrestin1-biased S1PR2 signaling in forebrain excitatory neurons • β-arrestin1-biased S1PR2 signaling recruits AP-1/CBP to increase Notch H3K27ac • Engineered  S. epidermidis  sustains  N -bc2S1P and extends rescue of CS-associated deficits
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Probiotic-driven microbiome remodeling is associated with coordinated immune and metabolic responses, improving growth and disease resistance in farmed tongue sole (Cynoglossus semilaevis)

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  Highlights • A probiotic-integrated semi-closed RAS protocol significantly improved growth (+0.08%/day) and survival (+6.9%) in Cynoglossus semilaevis. • Probiotic treatment reshaped the gut microbiota from Vibrio dominance to a Photobacterium-enriched community and altered quorum sensing pathways. • Metagenomics revealed enhanced CAZyme activity and nutrient metabolism potential in the probiotic group. • Multi-tissue transcriptomics demonstrated coordinated immune activation (NF-κB, IgA network) and JAK–STAT-mediated metabolic reprogramming. • Functional validation of ccl19 confirmed enhanced resistance against Vibrio harveyi, supporting an immune–metabolic crosstalk model.
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Targeting the US21 viroporin of human cytomegalovirus by calcium channel blockers as a new antiviral strategy

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  Highlights • CCBs bind the inner pore of the HCMV US21 viroporin and abolish pUS21-mediated ER Ca 2+  leakage. • CCBs inhibit HCMV replication. • CCBs prevent the cytobiological consequences of pUS21 expression. • CCBs treatment restores ER Ca 2+  levels to those of control cells. • CCBs do not affect basal intracellular Ca 2+  homeostasis.
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Predicting competition and substrate preferences for targeted microbiome alteration

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  Highlights • Translation measurements reveal how microbes allocate resources in communities • Functional profiles predict microbial competition and niche allocation • Predictions enable rational design of prebiotic and probiotic interventions • Interventions enable targeted modulation of complex microbiomes  in vitro  and  in vivo
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2'3'-cGAMP-induced membrane shearing promotes broad antiphage immunity

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  Highlights • Bacterial CD-NTase synthesizes 2'3'-cGAMP with mammalian cGAS-like enzymatic properties • 2'3'-cGAMP drives stepwise monomer-to-filament assembly of 3TM-SAVED family effectors • Filament assembly reconfigures TM helices and amphipathic hairpins to shear membranes • Vertical membrane shearing defines a distinct mode of membrane disruption
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Genomic and structural analysis of major antigenic proteins' variability in the human relapsing fever spirochete Borrelia persica

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  Highlights • Borrelia spirochetes have a unique mechanism to evade host immune system. • Borrelia persica has one chromosome, 12 linear and one circular plasmids. • Telomeric hairpin motifs were detected in the chromosome and linear plasmids. • Plasmid profiles remained stable after one year of continuous in vitro culture. • A Vlp common variable region kept the same external position across structures.
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The genome-scale sugar metabolic model from Neurospora crassa reveals lower gene redundancy than that of Aspergillus niger

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  Highlights • Neurospora crassa  has lower sugar metabolic gene redundancy than  Aspergillus niger • The mating type of  N. crassa  affects growth of sugar metabolic deletion strains • We present a strongly improved sugar metabolic model for  N. crassa
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