Mostly Microbes and Infection

Parabacteroides   is a   Gram-negative ,   anaerobic , non- spore -forming genus from the family   Tannerellaceae . First isolated from fecal specimen in 1933, type strain  Parabacteroides distasonis  was originally classified under the name  Bacteroides distasonis.  The strain was re-classified to form the new genus  Parabacteroides  in 2006.  Parabacteroides  currently comprise 21 phylogenetically, ecologically, and metabolically diverse species, 11 of which are listed in the taxonomic database 

  Highlights • Bacillus  sp. HC-9 controls honeysuckle leaf spot caused by  A. alternata. • Strain HC-9 inhibits eight phytopathogenic fungi with strong antifungal activity. • Strain HC-9 induces ROS and activates defense enzymes in honeysuckle leaves. • Field trials show HC-9 achieves up to 80.52% disease control efficiency.

  Highlights • Identification of the TamA protein as a highly conserved antigen across diverse  Klebsiella pneumoniae  strains, making it an ideal target for a broad-spectrum subunit vaccine. • Development and evaluation of a recombinant TamA protein vaccine that significantly enhances immune protection against a highly virulent  Klebsiella pneumoniae  infection in a murine model. • Demonstration of TamA-induced specific antibodies that effectively inhibit bacterial adherence to epithelial cells, thereby reducing bacterial load and pathological damage in vaccinated mice. • Significant survival benefit observed in TamA-vaccinated mice, with 80 % surviving over 10 days following a lethal dose challenge, compared to controls that survived less than 48 h. • Reduction in tissue bacterial loads and cytokine levels, along with mitigation of pathological changes in TamA-immunized mice, supporting the potential of TamA as a candidate vaccine for preventing  Klebsiel...

Chlamydiosen   sind durch verschiedene   Chlamydien   (bestimmte   Bakterienarten ) verursachte   Infektionskrankheiten . Sie betreffen das   Auge   (als   Trachom ), die   Atemwege   und die   Lunge   (als   Pneumonie ) und den   Urogenitaltrakt   (Harn- und Geschlechtsorgane) als   sexuell übertragene Krankheit .

  Highlights • Phospholipid diversity shapes membrane properties for growth and stress adaptation • Bacteria remodel phospholipid head groups and acyl chains to resist antimicrobials • Phospholipid and its metabolism serve as promising potential antibacterial targets

  Highlights • First systematic report of  Niallia taxi  BN5 with plant growth-promoting (PGP) activity, filling the  Niallia  PGP mechanism gap (previously studied for bioremediation/antibacterial functions). • Multi-omics (genome/transcriptome/qPCR) show BN5 has a complete trp operon for constitutive IAA biosynthesis (17.64 μg/mL); tryptophan regulates trp/motility genes. • BN5 enhances cucumber growth (height +24.29%, stem +11.23%), yield (+17.55%) and quality (vitamin C +17.2%, soluble sugar +37.41%) in pot/greenhouse trials. • BN5 reshapes rhizosphere microbiomes (enriches  Bacillus / Pseudomonas ) and elevates soil enzymes, providing a microbial resource for sustainable agriculture.

Many shots seem to have “off-target” benefits, such as lowering the risk of dementia, studies have found.

  Highlights • Distinct microbial communities are found in peritoneal dialysis (PD) and paired hemodialysis (HD) patients, despite similar microbiome diversity. • Serum p-cresyl sulfate (PCS) levels are significantly lower in PD patients compared to paired HD patients. • Altered microbial composition in PD patients, featuring more opportunistic pathogens and fewer beneficial bacteria, is linked to differences in metabolite profiles. • Fecal transplant experiments confirm that the HD-associated gut microbiota directly leads to higher PCS production. • Targeting the gut microbiota presents a potential therapeutic strategy to reduce uremic toxins in dialysis patients.

  Highlights • Salinity and nutrient availability affect the utilization and secretion of amino acids by deep biosphere microbial consortia • Anoxic deep biosphere enrichment cultures were compared to Desulfovibrio desulfuricans • Amino acids and organic acids were degraded under different nutrient conditions • Phe and Val degradation remained unaffected by changes in nutrient availability • Metabolic pathways involving Phe, Cys and Met were most prominent

  Highlights • Mixed reed and willow vegetation boosts denitrification more than monocultures. • nirK  denitrifiers are major drivers of N removal variation over  nirS . • Soil depth reshapes denitrifiers:  nirS  depth-sensitive,  nirK  nutrient-responsive. • Reed-willow boosts network complexity with depth to foster microbial interactions. • Bradyrhizobium, Sinorhizobiu m, and  Mesorhizobium  are key to mitigating N loss.

  Highlights • Pseudomonas, Escherichia and Prevotella dominated DFU assemblage across visits • Alcaligenes dominated healed DFU while Enterococcus was abundant in worsened DFU • High HbA1c favored Pseudomonas while Prevotella was abundant in lower HbA1c samples • Amputated DFUs had increased abundance of Escherichia and reduced Staphylococcus • Microbiome functional profiles in worsened DFU were enriched in virulence factors

  Highlights • Butyrolactol A synergizes with caspofungin against cryptococci and  Candida auris • BLA restores echinocandin efficacy by targeting the Apt1-Cdc50 flippase complex • Cryo-EM shows BLA traps the Apt1-Cdc50 phospholipid flippase in a dead-end state • BLA blocks lipid transport, disrupts membrane homeostasis, and boosts drug uptake

  Highlights • Viruses are largely overlooked in outdoor aeromicrobiological studies • Based on current knowledge in aeromicrobiology, a total of 10 21  virus particles are estimated to occupy clouds globally • Although very dilute, these may contribute to the atmospheric life cycle of microorganisms • Virus-bacteria interactions in clouds would have many implications in ecology and the Earth’s microbiome

  Highlights • Volume electron microscopy unveils intensive membrane networks in insect symbiotic cells • These tubenets are formed by intracellular bacteria, the insect’s nutritional symbionts • In situ  high spatial resolution chemical analysis shows tubenets’ enrichment in sugar • Bacterial tubenets increase the interface of exchange, maximizing nutrient acquisition

  Highlights • The  Borrelia  restriction-modification system (RMS) differs by species group. • The RMS affects the transformation efficiency of  Borrelia. • Increase in transformation efficiency observed with an in vitro methylated vector. • Transformation efficiency increased in low-passage  Borrelia  strains. • In vitro methylation can be a facilitating tool for  Borrelia  genetic manipulation.

  Highlights • Low concentration and high protection without disrupting microbial balance • Dual Protective Mechanism: targeting bacterial virulence and host Immune regulation • Multi-targeted inhibition of bacterial virulence • Elongation factor thermo unstable protein (EF-Tu) is identified as a key target of 5-iodoindole for first time • Superior application prospects for vibrio diseases control in aquaculture

  Highlights • Novel cyanobacterial genus Calyptranema fuscum gen. sp. nov. proposed. • Polyphasic approach combining morphology, phylogeny and genomics used. • ANI, AAI and POCP values confirm taxonomic novelty. • Pangenome analysis reveals genomic diversity within  Oculatellaceae .

  Melioidosis, caused by   Burkholderia pseudomallei , remains one of the most overlooked tropical infections—deadly, yet curable. Despite its discovery more than a century ago, this disease continues to hide behind the masks of tuberculosis, pneumonia, or sepsis, killing silently in regions where it is least recognized. In 2016, modelling studies estimated that South Asia accounts for nearly 44% of the global burden of melioidosis. ( Limmathurotsakul et al., 2016 ) Yet only a handful of countries—India, Sri Lanka, and Bangladesh—routinely diagnose and report human cases. In contrast, neighbouring nations such as Pakistan, Nepal, Bhutan, and Afghanistan have scarcely detected any cases, not because the disease is absent, but because it remains unseen — buried beneath diagnostic inexperience, limited laboratory capacity, and low clinical suspicion. The paradox is striking - while these countries share similar climatic, agricultural, and socioeconomic profiles, the ability to de...

  Highlights • Mercury (Hg) contamination restructured rice endophytic fungal communities, and higher Hg concentration decreased the richness and diversity of fungal endophytes, and the effect differed with tissues and development stages. • Grains endophytic fungal communities were distinct from roots/stalks/leaves, enriching rare taxa while lacking ubiquitous vegetative endophytes. • Two  Epicoccum nigrum  stains, DHJ7 and FZT214, reduced grains methylmercury by 44.67 % and 48.79 %, respectively. • 62.5 % of tested endophytes improved rice yield under Hg stress, and  Mucor  sp. DHJ19 and  Ceratorhiza  sp. CHJ27 improving yield 72.6–80.5 %.

  Highlights • Interconnected Antarctic brines show distinct bacterial and fungal communities • Fine-scale geochemistry and substrate context filter microbial assembly • B2 brine (hypersaline) shows halotolerant bacteria and many unclassified fungi • BCM brine shows permafrost/mineral imprint dominated by Patescibacteria and  Mrakia • BCR brine shows the highest diversity of soil- and glacier-derived microbial taxa